Original Research Article
Basic Medicine
Gholam Reza Goodarzi; Hamzeh Isazadeh
Abstract
Presenting a comprehensive model and identifying effective factors in the analysis of combat sports competitions was the goal of this article. For this purpose, the researchers referred to experts in the field of martial arts in Iran and collected information from them through in-depth interviews, and ...
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Presenting a comprehensive model and identifying effective factors in the analysis of combat sports competitions was the goal of this article. For this purpose, the researchers referred to experts in the field of martial arts in Iran and collected information from them through in-depth interviews, and then the data obtained from twenty interviews and documents were coded and analyzed. The research method was qualitative. The DIMTEL technique was used to find the model levels of the key factors of the analysis of combat sports competitions and any cause and effect relationships between the criteria. The results of the grouping of the codes showed fifteen components in four levels, which include the formation of separate training workshops for trainers, the use of skilled and educated people in this field, the intelligentization of the topic of analysis, and the formation of analysis teams for different age groups in the team. According to the results of the study, the presented model can be used as a sports analysis tool for the success of martial arts coaches in guiding and leading teams. In martial sports, the coach is one of the main and central figures. In this way, the analysis of martial sports competitions were extracted and confirmed, and the framework of the study was drawn and its suitability was confirmed. The purpose of this study is to provide a comprehensive model and identify effective factors in the analysis of martial arts competitions. The researchers referred to martial arts experts and collected information from them through interviews, and then the data from the interviews were coded.
Short Communication
Basic Medicine
Roohallah Yousefi
Abstract
Introduction: In beta-thalassemia, an imbalance in the production of beta subunits of hemoglobin leads to the oxidation and deposition of excess alpha-globin chains at the cell membrane, resulting in the hemolysis of erythrocytes and a disorder of erythropoiesis. Antioxidants, such as curcumin, may promote ...
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Introduction: In beta-thalassemia, an imbalance in the production of beta subunits of hemoglobin leads to the oxidation and deposition of excess alpha-globin chains at the cell membrane, resulting in the hemolysis of erythrocytes and a disorder of erythropoiesis. Antioxidants, such as curcumin, may promote this progression. This study aims to investigate the antioxidant effect of curcumin on hemolysate samples from patients with beta-thalassemia.Materials and methods: Pure curcumin was extracted and purified for use in studying its effect on the visual light absorbance of hemoglobin in hemolysate samples from beta-thalassemia patients compared to control samples. Changes in light absorbance at 540 and 700 nm wavelengths during exposure to curcumin were analyzed to examine the shift from oxyhemoglobin to deoxyhemoglobin.Results: Curcumin was found to dissolve rapidly and to a high degree in ethanol at 1 mg/ml, but did not dissolve in distilled water at the same concentration. The curcumin addition to the hemolysate sample of a patient with beta-thalassemia resulted in a decrease in the light absorbance of the sample at 540 nm wavelength, with minimal changes observed in the control sample.Conclusion: Curcumin deoxygenated the hemolysate samples from both the patient and control, causing hemoglobin precipitation to occur slowly. The study suggests a greater potential role for curcumin in deoxygenating hemoglobin in the hemolysate samples of beta-thalassemia patients compared to those of the normal control.
Mini-Review
Chemistry
Humphrey Sam Samuel; Francis-Dominic Makong Ekpan
Abstract
An invaluable method for assessing the surface morphology of a wide range of materials and samples, including those used in medical applications, is scanning electron microscopy (SEM). Scanning Electron Microscopy (SEM) has long been an indispensable tool in materials science and nanotechnology, providing ...
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An invaluable method for assessing the surface morphology of a wide range of materials and samples, including those used in medical applications, is scanning electron microscopy (SEM). Scanning Electron Microscopy (SEM) has long been an indispensable tool in materials science and nanotechnology, providing unparalleled insights into the microstructure and surface morphology of various materials. Recent advancements have extended the utility of SEM beyond traditional fields, including its promising applications in medicine and biomedical research. By leveraging the high-resolution imaging capabilities of SEM, researchers can delve deeper into the intricate structures of biological specimens, complexities of cellular architecture, tissue organization, and disease pathology. SEM provides more information on biocompatibility, surface interactions, and structural integrity of medical implants and devices, paving the way for enhanced diagnostic and therapeutic strategies. SEM has long been used to characterize the surface topography of cells and tissues. SEM has been utilized in Cell Surface Imaging, Drug delivery system, Tissue Microstructure, gastrointestinal, and cardiology to advance understanding of disease early stages. SEM can produce high-resolution pictures of inanimate and biological particles, allowing for a thorough examination of a range of medical applications.
Original Research Article
Basic Medicine
Roohallah Yousefi
Abstract
Introduction: Voxelotor, also known as GBT 440, binds to the alpha cleft in HbS tetramers and promotes the stability of the relaxed or oxygenated state of HbS. This process hinders the conformational change of the HbS tetramers into the deoxygenated state. Voxelotor prevents interactions between HbS ...
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Introduction: Voxelotor, also known as GBT 440, binds to the alpha cleft in HbS tetramers and promotes the stability of the relaxed or oxygenated state of HbS. This process hinders the conformational change of the HbS tetramers into the deoxygenated state. Voxelotor prevents interactions between HbS tetramers in the deoxygenated state, ultimately inhibiting the polymerization of HbS tetramers and resulting in significant clinical improvements, particularly in raising hemoglobin levels in patients. In this study, we have explored the use of herbal compound models, such as rosmarinic acid and compounds with similar structures that exhibit high binding affinity to Voxelotor's hemoglobin binding site.Materials and methods: The molecular model of hemoglobin (PDB: 5E83) was initially obtained from the RCSB PDB database. In addition, we collected 453 ligand models with structural similarity to rosmarinic acid from the PubChem database. To prepare these models for molecular docking, we utilized the Molegro Virtual Docker tool. Subsequently, we used the SwissADME web tool to predict the physicochemical properties and pharmacokinetics of these compounds. Results: We investigated the affinity and binding site of 453 compounds similar to rosmarinic acid on the hemoglobin model (PDB: 5E83). Our focus was on the alpha cleft between two alpha chains of the hemoglobin model (PDB: 5E83). The results showed that most compounds had molecular weights above 500 daltons, and some exhibited acceptable hydrophobicity. Furthermore, their solubility in aqueous solutions was good. None of the compounds were able to cross the blood-brain barrier or have gastrointestinal absorption. However, they did have varying inhibitory effects on CYP2C9 cytochromes. The skin penetration rate was generally low.
Conclusion: Through our study, we identified three compounds (CID: 162739375, CID: 141386569, and CID: 24015539) with promising potential for further research. These compounds demonstrated high binding affinity to the hemoglobin model, favorable dissolution and digestive absorption rates, as well as suitable hydrophobicity, making them ideal candidates for continued laboratory investigation
